An effective vaccine against Streptococcus pyogenes (Group A streptococcus, GAS) is urgently needed. Bacterial membrane vesicles (MVs) had been successfully used in vaccine formulations against Neisseria meningitidis1 and Streptococcus pneumoniae2. MVs have a great potential as a novel vaccine platform as they can activate the immune system, work as adjuvant and deliver antigens in their native conformation. In this study, we demonstrate that intranasal immunization with GAS-derived vesicles (GAS-MVs) elicits a strong humoral and cellular response in mice. Our results highlight the potential of GAS-derived vesicles as a novel antigen presenting platform that can aid in the development of a vaccine vs S. pyogenes.