Poster Presentation Lancefield International Symposium for Streptococci and Streptococcal Diseases 2025

Building laboratory capacity to advance Strep A vaccine development in Aotearoa New Zealand (#295)

Aimee Paterson 1 , Anna Vesty 1 2 , Natalie Lorenz 1 2 , Julie Bennett 1 2 3 , Anneka Anderson 1 , Rachel Webb 1 , Nicole J Moreland 1 2 , Reuben McGregor 1 2
  1. Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand
  2. Maurice Wilkins Centre for Biodiscovery, Auckland, New Zealand
  3. Department of Public Health, University of Otago, Wellington, New Zealand

Streptococcus A (Strep A) is a globally important pathogen causing over 500,000 deaths annually. In Aotearoa New Zealand Strep A inequitably effects Māori and Pacific Peoples and there are no licenced vaccines available. ’Rapua te mea ngaro ka tau’ (Rapua) brings together a multidisciplinary team to accelerate Strep A vaccine development for Aotearoa New Zealand, with workstreams focused on enhanced surveillance, community perceptions and clinical trial readiness. Embedded is the expansion of a laboratory dedicated to serological assays equipped with high-specification instruments, and the creation of a dedicated opsonophagocytic killing assay (OPKA) laboratory. The OPKA utilizes HL-60 cells differentiated into neutrophils with exogenous baby rabbit complement to quantify the ability of antibodies to promote phagocytosis and killing of Strep A bacteria, making it a critical tool for evaluating functional antibody responses for future vaccines. Recent key advancements with the OPKA in New Zealand include; 1) demonstration that the assay is reproducible, precise and accurate for sera from multiple species (rabbit, non-human primate and human), 2) application to better understand natural Strep A immune responses in clinical cohorts 3) developing standardised antibody reagents that demonstrate strain-specific killing in the OPKA and 4) establishing dedicated laboratory spaces to meet quality standards required for testing human biological specimens in clinical trials.  These advancements lay the foundation for a robust vaccine evaluation platform to support vaccine development.